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Document 3138
DOCN M94A3138
TI Seronegativation in an asymptomatic HIV1 infected patient.
DT 9412
AU Sousa A; Lourenco MH; Ferreira MO; Teles LC; Victorino RM; Faculdade de
Medicina, Lisboa, Portugal.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):145 (abstract no. PB0008). Unique
Identifier : AIDSLINE ICA10/94369437
AB OBJECTIVE: To report immunological and virological studies in an
asymptomatic HIV infected patient in wham seronegativation was
documented. METHODS: Two different ELISA methods were done as initial
tests for HIV1 antibodies followed by Western blot to identify
individual proteins sequentially done during the 6 years follow-up
period. Flow cytometry was used for quantitation of CD3+, CD8+, CD45RO+,
CD45RA+, cells in peripheral blood. Lymphocyte proliferative responses
to mitogens was measured by 3H thymidine incorporation in peripheral
blood mononuclear cells cultured in triplicate. Similarly responses to
HIV1 and HIV2 recombinant proteins in three different concentrations (gp
120, gp 105, p 24 and p 26 provided by the MRC-U.K.) were studied using
24 replicates (24 culture wells for each concentration of protein).
RESULTS: The patient was a 48 year old female who was tested for HIV in
a blood bank and was found to have a clearly positive ELISA test and
antibodies to all HIV1 strutural proteins on two different occasions (gp
160, gp 110, gp 41, p 24, p 18). Although the patient lived in Portugal,
her sexual partner had lived long periods in Central Africa. She was
referred to an HIV clinic where she was followed for 6 years. A
progressive loss of antibodies to the different proteins was recorded in
the sequential Western blots (first to envelope proteins and finally to
core). She has presently no antibodies to any of the HIV proteins.
Levels of CD4+, CD8+, CD45RO+, CD45RA+ cells were normal along the
follow-up. Studies on the T helper responses to recombinant HIV1
proteins (core and envelope) will be presented. CONCLUSION: This case
illustrates the possibility of seronegativation in HIV1 infection and
this will be discussed considering the epidemiological data and the
results of the immunological studies.
DE Case Report Enzyme-Linked Immunosorbent Assay/METHODS Female Flow
Cytometry Human HIV Antibodies/*BLOOD HIV Seronegativity/*IMMUNOLOGY
HIV Seropositivity/*IMMUNOLOGY *HIV-1 Lymphocyte Transformation
Lymphocytes/IMMUNOLOGY Middle Age T-Lymphocytes,
Helper-Inducer/IMMUNOLOGY MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).